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SP delivery induced HO in Achilles at week 6 post-operation according to the <t>microCT</t> scans. Co-delivery of SP and CGRP did not exhibit any HO. (A) Representative microCT images of the treated legs. Scale bars measure 5mm. Arrows point to the HO nodules around the Achilles. (B) HO around the calcaneus and the Achilles. Bars represent the average HO volume ± SEM. n = 6 per group. (C) HO around the calcaneus and the Achilles. Bars represent the bone volume (BV)/total volume (TV) ratio ± SEM. Control animals did not show any HO around the tendon, but the micro hole drilled through the calcaneus was healed. SP induced significant HO (p < 0.001) around the Achilles, while CGRP did not have a significant effect (p = 0.9 compared to the controls, p < 0.01 compared to SP-delivered tendons). Co-delivery of SP and CGRP did not induce HO (p μ 0.001 compared to SP-delivered tendons).
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SP delivery induced HO in Achilles at week 6 post-operation according to the microCT scans. Co-delivery of SP and CGRP did not exhibit any HO. (A) Representative microCT images of the treated legs. Scale bars measure 5mm. Arrows point to the HO nodules around the Achilles. (B) HO around the calcaneus and the Achilles. Bars represent the average HO volume ± SEM. n = 6 per group. (C) HO around the calcaneus and the Achilles. Bars represent the bone volume (BV)/total volume (TV) ratio ± SEM. Control animals did not show any HO around the tendon, but the micro hole drilled through the calcaneus was healed. SP induced significant HO (p < 0.001) around the Achilles, while CGRP did not have a significant effect (p = 0.9 compared to the controls, p < 0.01 compared to SP-delivered tendons). Co-delivery of SP and CGRP did not induce HO (p μ 0.001 compared to SP-delivered tendons).

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Article Title: Crosstalk Between Substance P and Calcitonin Gene-Related Peptide During Heterotopic Ossification in Murine Achilles Tendon

doi: 10.1002/jor.23833

Figure Lengend Snippet: SP delivery induced HO in Achilles at week 6 post-operation according to the microCT scans. Co-delivery of SP and CGRP did not exhibit any HO. (A) Representative microCT images of the treated legs. Scale bars measure 5mm. Arrows point to the HO nodules around the Achilles. (B) HO around the calcaneus and the Achilles. Bars represent the average HO volume ± SEM. n = 6 per group. (C) HO around the calcaneus and the Achilles. Bars represent the bone volume (BV)/total volume (TV) ratio ± SEM. Control animals did not show any HO around the tendon, but the micro hole drilled through the calcaneus was healed. SP induced significant HO (p < 0.001) around the Achilles, while CGRP did not have a significant effect (p = 0.9 compared to the controls, p < 0.01 compared to SP-delivered tendons). Co-delivery of SP and CGRP did not induce HO (p μ 0.001 compared to SP-delivered tendons).

Article Snippet: Three-dimensional reconstruction of the lateral projections were performed using the Scanco software from the raw files and the resulting volumes were processed with the Scanco microCT 3D morphometry and densitometry software, operated in an open VMS environment.

Techniques: